Coding

Part:BBa_K2984029:Design

Designed by: Dimitri Schumacher   Group: iGEM19_Humboldt_Berlin   (2019-10-16)


L1c-PsaD-GLE-PETase-YFP-SP20-RBCS2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1775
    Illegal PstI site found at 1989
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1314
    Illegal PstI site found at 1775
    Illegal PstI site found at 1989
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal XhoI site found at 1038
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1775
    Illegal PstI site found at 1989
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1775
    Illegal PstI site found at 1989
    Illegal NgoMIV site found at 2466
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This construct was designed on a level 1 c backbone (BBa_K2984002). Together with L1a (BBa_K2984003) and L1b (BBa_K2984005), a level 2 construct can be built for multiple gene insertion.


Source

The construct was built out of 6 different level 0 plasmids using the MoClo standard.

References